publications

Resources

Our collaborators

Prof. Maria Spies (University of Iowa, Carver College of Medicine)
Dr. Ann Zeuner (Department of Oncology and Molecular Medicine, ISS, Rome)
Dr. Bob Brosh (National Institute on Aging, NIH Baltimore)
Prof. Iain Morgan (Virginia Commonwealth University)
Dr. Ilio Vitale (IIGM, Candiolo, Italy)
Prof. Daniela Barilà (University of Roma, Tor Vergata)
Prof. Emma Di Carlo (University G D'Annunzio, Chieti)
Prof. Elena Petricci (University of Siena)
Prof. Achille Pellicioli (University of Milan)

publications of the GSG

(most recent)

Phosphorylation-Dependent Association of WRN with RPA is Required for Recovery of Replication Forks Stalled at Secondary DNA Structures.

Noto A, Valenzisi P, Di Feo F, Fratini F, Kulikowicz T, Sommers JA, Palermo V, Semproni M, Crescenzi M, Brosh RM Jr, Franchitto A, Pichierri P.
Nature communications, 16(1), 997. https://doi.org/10.1038/s41467-025-55958-z

WRNIP1 prevents transcription-associated genomic instability.

Valenzisi P, Marabitti V, Pichierri P, Franchitto A.
eLife12:RP89981 https://doi.org/10.7554/eLife.89981.1

Switch-Like Phosphorylation of WRN Integrates End-Resection with RAD51 Metabolism at Collapsed Replication Forks.

Palermo V, Malacaria E, Semproni M, Camerini S, Casella M, Perdichizzi B, Valenzisi P, Sanchez M, Marini F, Pellicioli A, Franchitto A, Pichierri P.
Nucleic Acids Res. 2024 Sep 24:gkae807. doi: 10.1093/nar/gkae807. Online ahead of print. PMID: 39315694

Phosphorylation status of MUS81 is a modifier of Olaparib sensitivity in BRCA2-deficient cells.
Blandino F, Malacaria E, Figlioli C, Noto A, Pugliese GM, Franchitto A, Pichierri P.
Nucleic Acids Res. 2023 May 31:gkad470. doi: 10.1093/nar/gkad470. Epub ahead of print. PMID: 37254810.
Therapeutic disruption of RAD52-ssDNA complexation via novel drug-like inhibitors.
Bhat DS, Malacaria E, Biagi LD, Razzaghi M, Honda M, Hobbs KF, Hengel SR, Pichierri P, Spies MA, Spies M.
NAR Cancer. 2023 May 1;5(2):zcad018. doi: 10.1093/narcan/zcad018. PMID: 37139244; PMCID: PMC10150327.
RAD52 prevents accumulation of Polα-dependent replication gaps at perturbed replication forks in human cells.
Di Biagi L, Malacaria E, Aiello FA, Valenzisi P, Marozzi G, Franchitto A, Pichierri P.
bioRxiv [Preprint]. 2023 Apr 12:2023.04.12.536536. doi: 10.1101/2023.04.12.536536. PMID: 37090680; PMCID: PMC10120653.
R-Loop-Associated Genomic Instability and Implication of WRN and WRNIP1.
Marabitti V, Valenzisi P, Lillo G, Malacaria E, Palermo V, Pichierri P, Franchitto A.
Int J Mol Sci. 2022 Jan 28;23(3):1547. doi: 10.3390/ijms23031547. PMID: 35163467; PMCID: PMC8836129.
Control of replication stress and mitosis in colorectal cancer stem cells through the interplay of PARP1, MRE11 and RAD51.
Manic G, Musella M, Corradi F, Sistigu A, Vitale S, Soliman Abdel Rehim S, Mattiello L, Malacaria E, Galassi C, Signore M, Pallocca M, Scalera S, Goeman F, De Nicola F, Guarracino A, Pennisi R, Antonangeli F, Sperati F, Baiocchi M, Biffoni M, Fanciulli M, Maugeri-Saccà M, Franchitto A, Pichierri P, De Maria R, Vitale I.
Cell Death Differ. 2021 Jul;28(7):2060-2082. doi: 10.1038/s41418-020-00733-4. Epub 2021 Feb 2. PMID: 33531658; PMCID: PMC8257675.
Using a Human Papillomavirus Model to Study DNA Replication and Repair of Wild Type and Damaged DNA Templates in Mammalian Cells.
Das D, Bristol ML, Pichierri P, Morgan IM.
Int J Mol Sci. 2020 Oct 13;21(20):7564. doi: 10.3390/ijms21207564. PMID: 33066318; PMCID: PMC7589113.
Physiological and Pathological Roles of RAD52 at DNA Replication Forks.
Malacaria E, Honda M, Franchitto A, Spies M, Pichierri P.
Cancers (Basel). 2020 Feb 10;12(2):402. doi: 10.3390/cancers12020402. PMID: 32050645; PMCID: PMC7072239.
Checkpoint Defects Elicit a WRNIP1-Mediated Response to Counteract R-Loop-Associated Genomic Instability.
Marabitti V, Lillo G, Malacaria E, Palermo V, Pichierri P, Franchitto A.
Cancers (Basel). 2020 Feb 7;12(2):389. doi: 10.3390/cancers12020389. PMID: 32046194; PMCID: PMC7072626.
Inducible SMARCAL1 knockdown in iPSC reveals a link between replication stress and altered expression of master differentiation genes.
Pugliese GM, Salaris F, Palermo V, Marabitti V, Morina N, Rosa A, Franchitto A, Pichierri P.
Dis Model Mech. 2019 Oct 17;12(10):dmm039487. doi: 10.1242/dmm.039487. PMID: 31515241; PMCID: PMC6826020.
RAD51 and mitotic function of mus81 are essential for recovery from low-dose of camptothecin in the absence of the WRN exonuclease.
Aiello FA, Palma A, Malacaria E, Zheng L, Campbell JL, Shen B, Franchitto A, Pichierri P.
Nucleic Acids Res. 2019 Jul 26;47(13):6796-6810. doi: 10.1093/nar/gkz431. PMID: 31114910; PMCID: PMC6648349.
Rad52 prevents excessive replication fork reversal and protects from nascent strand degradation.
Malacaria E, Pugliese GM, Honda M, Marabitti V, Aiello FA, Spies M, Franchitto A, Pichierri P.
Nat Commun. 2019 Mar 29;10(1):1412. doi: 10.1038/s41467-019-09196-9. Erratum in: Nat Commun. 2019 May 1;10(1):2023. PMID: 30926821; PMCID: PMC6441034.
Werner Helicase Control of Human Papillomavirus 16 E1-E2 DNA Replication Is Regulated by SIRT1 Deacetylation.
Das D, Bristol ML, Smith NW, James CD, Wang X, Pichierri P, Morgan IM.
mBio. 2019 Mar 19;10(2):e00263-19. doi: 10.1128/mBio.00263-19. Erratum in: mBio. 2019 Aug 13;10(4): PMID: 30890607; PMCID: PMC6426601.
ATM pathway activation limits R-loop-associated genomic instability in Werner syndrome cells.
Marabitti V, Lillo G, Malacaria E, Palermo V, Sanchez M, Pichierri P, Franchitto A.
Nucleic Acids Res. 2019 Apr 23;47(7):3485-3502. doi: 10.1093/nar/gkz025. PMID: 30657978; PMCID: PMC6468170.
Interaction of the Warsaw breakage syndrome DNA helicase DDX11 with the replication fork-protection factor Timeless promotes sister chromatid cohesion.
Cortone G, Zheng G, Pensieri P, Chiappetta V, Tatè R, Malacaria E, Pichierri P, Yu H, Pisani FM.
PLoS Genet. 2018 Oct 10;14(10):e1007622. doi: 10.1371/journal.pgen.1007622. PMID: 30303954; PMCID: PMC6179184.
Phosphorylation by CK2 regulates MUS81/EME1 in mitosis and after replication stress.
Palma A, Pugliese GM, Murfuni I, Marabitti V, Malacaria E, Rinalducci S, Minoprio A, Sanchez M, Mazzei F, Zolla L, Franchitto A, Pichierri P.
Nucleic Acids Res. 2018 Jun 1;46(10):5109-5124. doi: 10.1093/nar/gky280. PMID: 29850896; PMCID: PMC6007509.
SLX4 Prevents GEN1-Dependent DSBs During DNA Replication Arrest Under Pathological Conditions in Human Cells.
Malacaria E, Franchitto A, Pichierri P.
Sci Rep. 2017 Mar 14;7:44464. doi: 10.1038/srep44464. PMID: 28290553; PMCID: PMC5349550.
Way out/way in: How the relationship between WRN and CDK1 may change the fate of collapsed replication forks.
Palermo V, Rinalducci S, Sanchez M, Grillini F, Franchitto A, Pichierri P.
Mol Cell Oncol. 2016 Dec 9;4(1):e1268243. doi: 10.1080/23723556.2016.1268243. PMID: 28197541; PMCID: PMC5286962.
WRNIP1: A new guardian of genome integrity at stalled replication forks.
Leuzzi G, Marabitti V, Pichierri P, Franchitto A.
Mol Cell Oncol. 2016 Aug 3;3(5):e1215777. doi: 10.1080/23723556.2016.1215777. PMID: 27857978; PMCID: PMC5068177.
CDK1 phosphorylates WRN at collapsed replication forks.
Palermo V, Rinalducci S, Sanchez M, Grillini F, Sommers JA, Brosh RM Jr, Zolla L, Franchitto A, Pichierri P.
Nat Commun. 2016 Sep 16;7:12880. doi: 10.1038/ncomms12880. PMID: 27634057; PMCID: PMC5028418.
WRNIP1 protects stalled forks from degradation and promotes fork restart after replication stress.
Leuzzi G, Marabitti V, Pichierri P, Franchitto A.
EMBO J. 2016 Jul 1;35(13):1437-51. doi: 10.15252/embj.201593265. Epub 2016 May 30. PMID: 27242363; PMCID: PMC4931187.
The WRN exonuclease domain protects nascent strands from pathological MRE11/EXO1-dependent degradation.
Iannascoli C, Palermo V, Murfuni I, Franchitto A, Pichierri P.
Nucleic Acids Res. 2015 Nov 16;43(20):9788-803. doi: 10.1093/nar/gkv836. Epub 2015 Aug 14. PMID: 26275776; PMCID: PMC4787784.